Screening and Validation of SSR Molecular Markers for Identification of Downy Mildew Resistance in Intraspecific Hybrid F1 Progeny (V. vinifera)

Downy mildew is a major disease that severely restricts the healthy and sustainable development of global grape wine industry, so there significant interest in breeding high-quality disease-resistant varieties. In this study, hybridization was carried out between two four varieties for eight hybrid combinations. The downy resistance 6 parents 350 F1 progeny determined by leaf disc inoculation test, frequency distribution grades analyzed different populations. Agarose gel electrophoresis identification were used to screen SSR molecular markers tested hybrids mildew. results showed minor disease-resistance genes V. vinifera main determinant variation susceptibility among inherited quantitative trait controlled multiple genes. Intraspecific can be applied breed suitable intermediate materials or new types resistance. screening verification simple-sequence repeats (SSR) as revealed UDV-304, UDV-305, UDV-737 could standard Ecolly Cabernet Sauvignon, UDV-305 Meili VMC8g9 Dunkelfelder. selected study high-quality, intraspecific recurrent selection vinifera. screened utilized rapid batch combinations facilitate marker-assisted breeding.